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MLT (ASCP) Exam
Healthcare & Nursing

Medical Laboratory Technician — MLT(ASCP) Certification Exam — ASCP Board of Certification

The MLT(ASCP) certification exam from the ASCP Board of Certification tests entry-level medical laboratory technicians in blood banking, urinalysis and body fluids, chemistry, hematology, immunology, microbiology and laboratory operations. It is a computer adaptive test of 100 multiple-choice questions in 2 hours 30 minutes. SummitCert's portal provides explained practice questions, flashcards, a timed 100-question mock and progress tracking by content area.

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350+ exam-style practice questions quiz pool

Your full practice pool — 350 questions total, written to mirror the real exam blueprint, with full answer explanations for every question.

7 exam domains covered

Including Blood Banking, Urinalysis and Other Body Fluids, Chemistry, and more.

Full mock exam — 100 questions, 150-min timed

A complete, realistic exam-day simulation with the same question count and time limit as the real thing.

1000+ flashcards

Covering every key term, concept, and framework you're expected to know.

244+ curated video lessons

Hand-picked walkthroughs and explainers organized by topic.

40+ diagrams & visual references

Visual breakdowns of the frameworks and models the exam tests you on.

19+ key formulas & quick-reference sheet

The exact formulas and calculations you need, ready to review before test day.

134+ term glossary

A searchable glossary so unfamiliar terminology never slows you down.

Progress tracking & study planner

See your accuracy by domain, spot weak areas early, and follow a built-in study schedule.

Sage — your personalized AI study companion

Tailored to this exam: it knows your progress and weak areas, remembers your earlier chats, and picks up where you left off on your other devices.

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Topics you'll drill

7 core topics pulled straight from the real question bank.

  • Blood Banking
  • Urinalysis and Other Body Fluids
  • Chemistry
  • Hematology
  • Immunology
  • Microbiology
  • Laboratory Operations

MLT (ASCP) Exam exam requirements & cost

Straight from the certifying body — separate from what we charge for the study portal.

Eligibility
ASCP BOC accepts several eligibility routes based on education and clinical laboratory experience; confirm the route that fits you on the ASCP BOC site before you apply
Real exam cost
Set by ASCP BOC and differs for U.S. and international applicants; confirm the current application fee with ASCP BOC
Format
100 multiple-choice questions · 2 hours 30 minutes · computer adaptive · scaled score 100 to 999 · four options per question

The ASCP BOC content guideline (revised September 25, 2025) describes the MLT exam as 100 multiple-choice questions with four options and one best answer, delivered by computer adaptive testing in 2 hours 30 minutes; the portal mock uses 100 questions and 150 minutes. Content areas and shares: Blood Banking 17 percent, Urinalysis and Other Body Fluids 7, Chemistry 23, Hematology 23, Immunology 7, Microbiology 17 and Laboratory Operations 6. ASCP BOC reports a scaled score from 100 to 999 with a minimum passing score of 400; no set number or percentage of correct answers is required, and the exam includes unscored field-test questions whose number is not published. ASCP BOC reports a scaled score of 100 to 999 and the minimum passing score is 400; the exam is adaptive, so there is no fixed number of correct answers. The portal mock shows a percent score only as a pacing and knowledge self-check, not a prediction. ASCP BOC sets the application fee, which differs for U.S. and international applicants and can change, so no amount is quoted here; confirm the current fee on the ASCP BOC site before you apply. The content outline spreads questions across 7 areas; the largest are Chemistry (23.0%); Hematology (23.0%); Blood Banking (17.0%). The full list of areas and weights is on the portal exam page. ASCP BOC accepts several eligibility routes based on education and clinical laboratory experience; confirm the route that fits you on the ASCP BOC site before you apply.

MLT (ASCP) Exam exam blueprint

Real domain weighting from the certifying body's own published exam syllabus.

  • Blood Banking17%
  • Urinalysis and Other Body Fluids7%
  • Chemistry23%
  • Hematology23%
  • Immunology7%
  • Microbiology17%
  • Laboratory Operations6%
Last verified: October 2026·Exam blueprint: Compiled on October 30, 2026 from the MLT(ASCP) and MLT(ASCPi) examination content guideline revised September 25, 2025 (seven content areas and shares, 100 questions, adaptive delivery) and the ASCP BOC examination process pages (scaled score and pass point). Fees are not quoted because published figures differ; confirm them with ASCP BOC.·Primary source: ASCP BOC: MLT and MLT(ASCPi) content guideline ↗
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Common questions

The ASCP BOC content guideline (revised September 25, 2025) describes the MLT exam as 100 multiple-choice questions with four options and one best answer, delivered by computer adaptive testing in 2 hours 30 minutes; the portal mock uses 100 questions and 150 minutes. Content areas and shares: Blood Banking 17 percent, Urinalysis and Other Body Fluids 7, Chemistry 23, Hematology 23, Immunology 7, Microbiology 17 and Laboratory Operations 6. ASCP BOC reports a scaled score from 100 to 999 with a minimum passing score of 400; no set number or percentage of correct answers is required, and the exam includes unscored field-test questions whose number is not published. Format: 100 questions in 2 hours 30 minutes, computer adaptive; the portal mock is a fixed 100-question set in 150 minutes.

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